KMID : 0191120070220050883
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Journal of Korean Medical Science 2007 Volume.22 No. 5 p.883 ~ p.890
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Microarray Analysis of Thyroid Stimulating Hormone, Insulin-Like Growth Factor-1, and Insulin-Induced Gene Expression in FRTL-5 Thyroid Cells
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Lee You-Jin
Kim Seong-Yeon Park Kyong-Soo Shin Chan-Soo Park Do-Joon Cho Bo-Youn Lee Hong-Kyu Park Young-Joo
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Abstract
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To determine which genes are regulated by thyroid stimulating hormone (thyrotropin, TSH), insulin and insulin-like growth factor-1 (IGF-1) in the rat thyroid, we used the microarray technology and observed the changes in gene expression. The expressions of genes for bone morphogenetic protein 6, the glucagon receptor, and cyclin D1 were increased by both TSH and IGF-1; for cytochrome P450, 2c37, the expression was decreased by both. Genes for cholecystokinin, glucuronidase, beta, demethyl-Q 7, and cytochrome c oxidase, subunit VIIIa, were up-regulated; the genes for ribosomal protein L37 and ribosomal protein L4 were down-regulated by TSH and insulin. However, there was no gene observed to be regulated by all three: TSH, IGF-1, and insulin molecules studied. These findings suggest that TSH, IGF-1, and insulin stimulate different signal pathways, which can interact with one another to regulate the proliferation of thyrocytes, and thereby provide additional influence on the process of cellular proliferation.
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KEYWORD
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Oligonucleotide Array Sequence Analysis, Gene Expression, Thyroid, Thyrotropin, Insulin, Insulin-Like Growth Factor 1
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